2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
1 Department of Bioengineering, University of California, Berkeley, CA, 94720, USA
2 QB3: California Institute for Quantitative Biological Research, University of California, Berkeley, CA, 94720, USA
3 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA
4 Current address: Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA
Journal of Biological Engineering 2013, 7:2 doi:10.1186/1754-1611-7-2Published: 10 January 2013
There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.