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2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Mariana Leguia123, Jennifer AN Brophy1, Douglas Densmore14, Angel Asante1 and J Christopher Anderson123*

Author Affiliations

1 Department of Bioengineering, University of California, Berkeley, CA, 94720, USA

2 QB3: California Institute for Quantitative Biological Research, University of California, Berkeley, CA, 94720, USA

3 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

4 Current address: Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA

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Journal of Biological Engineering 2013, 7:2  doi:10.1186/1754-1611-7-2

Published: 10 January 2013


There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

2ab reaction; Automated assembly; DNA fabrication; Synthetic biology