Email updates

Keep up to date with the latest news and content from Journal of Biological Engineering and BioMed Central.

Open Access Highly Accessed Methodology

2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Mariana Leguia123, Jennifer AN Brophy1, Douglas Densmore14, Angel Asante1 and J Christopher Anderson123*

Author Affiliations

1 Department of Bioengineering, University of California, Berkeley, CA, 94720, USA

2 QB3: California Institute for Quantitative Biological Research, University of California, Berkeley, CA, 94720, USA

3 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

4 Current address: Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA

For all author emails, please log on.

Journal of Biological Engineering 2013, 7:2  doi:10.1186/1754-1611-7-2

Published: 10 January 2013

Abstract

There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

Keywords:
2ab reaction; Automated assembly; DNA fabrication; Synthetic biology