Temperature dependence of ssrA-tag mediated protein degradation
1 MIT Synthetic Biology Center, Massachusetts Institute of Technology, 500 Technology Square, NE47-235, Cambridge, MA 02139 USA
2 School of Biological Sciences, University of Bristol, Bristol BS8 1UG, UK
3 Department of Engineering Mathematics, University of Bristol, Bristol BS8 1TR, UK
4 Department of Systems and Computer Engineering, University of Naples Federico II, Via Claudio 21, Napoli, 80125, Italy
5 School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
Journal of Biological Engineering 2012, 6:10 doi:10.1186/1754-1611-6-10Published: 23 July 2012
Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be subjected to variable conditions, and be required to display precise, quantitative dynamics, which will be more susceptible to environmental changes than the general qualitative dynamics focussed on so far.