Figure 5.

Concurrent mRNA inactivation and protein degradation in a cell-free system. (A) End-point measurement of deGFP expression (1 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 or 1 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-SsrA-T500). 1) no degradation (0 nM MazF, deGFP), 2) mRNA inactivation (36 nM MazF, deGFP), 3) protein degradation (0 nM MazF, deGFP-SsrA), 4) expected protein production with mRNA inactivation (36 nM MazF) and protein degradation (deGFP-SsrA), 5) protein production measured with mRNA inactivation (36 nM MazF) and protein degradation (deGFP-SsrA). (B) Kinetics of expression for the different cases (1 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 or 1 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-SsrA-T500).