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Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
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Corresponding authors: Jonghyeon Shin shin@physics.umn.edu - Vincent Noireaux noireaux@umn.edu
University of Minnesota, 116 Church Street S.E., Minneapolis, MN 55455, USA
Journal of Biological Engineering 2010, 4:8 doi:10.1186/1754-1611-4-8
Published: 24 June 2010Additional files
Additional file 1:
Supplementary information for Shin and Noireaux "Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70". supplementary information includes a list of the sequences, data on expression optimization (DTT, tRNA, amino acids, nucleotides), expression using pure E. coli RNAP for transcription, plasmid maps, expression of eGFP and eGFP-Del6-229 in E. coli.
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