Research
Insulation of a synthetic hydrogen metabolism circuit in bacteria
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* Corresponding author: Pamela A Silver pamela_silver@hms.harvard.edu
1 Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
2 Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
Journal of Biological Engineering 2010, 4:3 doi:10.1186/1754-1611-4-3
Published: 25 February 2010Additional files
Additional file 1:
Supplementary information for Agapakis et. al. "Insulation of a synthetic hydrogen metabolism circuit in bacteria." Supplementary information includes nucleotide sequences for commercially synthesized genes, a table listing all oligonucleotide primers used, sequence information for the mutated expression vectors, protein sequence alignment of hydrogenase enzymes used in the hydrogen metabolism circuit, domain structure of electron transfer proteins deleted in the study, and nucleotide alignment of Chlamydomonas reinhardtii HydA1 and HydA2.
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