Insulation of a synthetic hydrogen metabolism circuit in bacteria

doi:10.1186/1754-1611-4-3

Journal of Biological Engineering

Volume 4

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Agapakis CM
Ducat DC
Boyle PM
Wintermute EH
Way JC
Silver PA

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Boyle PM
Wintermute EH
Way JC
Silver PA
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Insulation of a synthetic hydrogen metabolism circuit in bacteria

Christina M Agapakis¹ , Daniel C Ducat¹^,2 , Patrick M Boyle¹ , Edwin H Wintermute¹ , Jeffrey C Way² and Pamela A Silver¹^,2

¹Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA

²Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA

author email corresponding author email

Journal of Biological Engineering 2010, 4:3doi:10.1186/1754-1611-4-3


Published:	25 February 2010

Additional files

Additional file 1:

Supplementary information for Agapakis et. al. "Insulation of a synthetic hydrogen metabolism circuit in bacteria." Supplementary information includes nucleotide sequences for commercially synthesized genes, a table listing all oligonucleotide primers used, sequence information for the mutated expression vectors, protein sequence alignment of hydrogenase enzymes used in the hydrogen metabolism circuit, domain structure of electron transfer proteins deleted in the study, and nucleotide alignment of Chlamydomonas reinhardtii HydA1 and HydA2.

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