Additional file 1.pZS2-123 annotated sequence. Sequence of the three-color reporter construct in a 6,806 base-pair plasmid containing a Kanamycin resistance gene and an SC101 origin of replication. Sequence +1 begins at the single NotI restriction site at the beginning of the 3,518 base-pair three color reporter scaffold (the three color scaffold sequence ends at the NheI site). All genetic elements described in the Methods section are annotated as sequence features, along with the primers used for sequence verification. Format: GB Size: 172KB Download file Cox et al. Journal of Biological Engineering 2010 4:10 doi:10.1186/1754-1611-4-10 |