Research
A synthetic three-color scaffold for monitoring genetic regulation and noise
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* Corresponding author: Michael B Elowitz melowitz@caltech.edu
1 Division of Biology, California Institute of Technology, Pasadena, CA, USA
2 Department of Engineering and Applied Science, California Institute of Technology, Pasadena, CA, USA
3 RIKEN Systems and Structural Biology Center, Yokohama, Japan
4 Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
5 Howard Hughes Medical Institute, California Institute of Technology 1200 E. California Blvd. M/C 114-96 Pasadena, CA 91125, USA
Journal of Biological Engineering 2010, 4:10 doi:10.1186/1754-1611-4-10
Published: 21 July 2010Additional files
Additional file 1:
pZS2-123 annotated sequence. Sequence of the three-color reporter construct in a 6,806 base-pair plasmid containing a Kanamycin resistance gene and an SC101 origin of replication. Sequence +1 begins at the single NotI restriction site at the beginning of the 3,518 base-pair three color reporter scaffold (the three color scaffold sequence ends at the NheI site). All genetic elements described in the Methods section are annotated as sequence features, along with the primers used for sequence verification.
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Additional file 2:
Figure S1: Total genetic noise is controlled by induction. The total genetic noise, calculated as the standard error divided by the mean, is plotted for each of the conditions in Figure 2. Cyan corresponds to noise in cfp, yellow to noise in yfp, and red to noise in rfp. In each case, the noise is maximal in the fully induced state. The noise of each color is only affected by the associated inducer(s): aTc for cfp, IPTG for yfp, and both IPTG and L-ara for rfp.
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Timelapse movie of 3 color network, yellow and cyan channels.
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Timelapse movie of 3 color network, yellow and red channels.
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