DNA sequence verification of HPP solutions. Three yellow fluorescent colonies from each of the three recombination plates were used for plasmid preparation and DNA sequencing. The number of ABC and ABC' solution genotypes found for each of the starting constructs is listed. The order and orientations of GFP (green) and RFP (red) gene halves for each of the starting constructs and solutions is illustrated.
Baumgardner et al. Journal of Biological Engineering 2009 3:11 doi:10.1186/1754-1611-3-11