Table 1

Two-pancake permutations and their phenotypes
BPP Plasmid constructa
 Perm.b
 mRFP expression on amp, IPTGc
 Growth on tet, amp, IPTGd


expected
 observed
 expected
 observed
RR-hixC-pLac-hixC-RBS-tetA(C)-hixC
 (1, 2)
 -
 -
 +
 weak
RR-hixC-tetA(C)rev-RBSrev-hixC-pLacrev-hixC
 (-2, -1)
 +
 -
 +
 +
RR-hixC-pLac-hixC-tetA(C)rev-RBSrev-hixC
 (1, -2)
 -
 -
 -
 weak
RR-hixC-RBS-tetA(C)-hixC-pLacrev-hixC
 (2, -1)
 +
 -
 -
 -
RR-hixC-pLacrev-hixC-RBS-tetA(C)-hixC
 (-1, 2)
 +
 +
 -
 +
RR-hixC-tetA(C)rev-RBSrev-hixC-pLac-hixC
 (-2, 1)
 -
 -
 -
 +
RR-hixC-pLacrev-hixC-tetA(C)rev-RBSrev-hixC
 (-1, -2)
 +
 +
 -
 -
RR-hixC-RBS-tetA(C)-hixC-pLac-hixC
 (2, 1)
 -
 -
 -
 +

a Two-pancake BPP constructs in plasmid pSB4A3.

b Signed permutation corresponding to each BPP construct.

c Cells carrying a BPP plasmid were selected on ampicillin (amp) plates. IPTG-induced expression of mRFP was visually detected as fluorescent red color (+).

d Serial dilutions of colonies showed normal (+), weak, or no (-) growth on tetracycline (tet) upon IPTG-induced tetA(C) expression.

Haynes et al. Journal of Biological Engineering 2008 2:8   doi:10.1186/1754-1611-2-8
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