Table 1

Endonuclease sites targeted for removal from BioBrick vector parts.

Endonuclease
Description

EcoRI, XbaI, SpeI, PstI
BioBrick restriction site
ApoI, MfeI
Produces compatible ends to EcoRI
AvrII, NheI
Produces compatible ends to XbaI and SpeI
NsiI SbfI
Produces compatible ends to PstI
AarI, AcuI, BbsI, BciVI, BfuAI, BmrI, BsaI, BsgI, BsmBI, BsmI, BspMI, BsrDI, BtgZI, EarI, EcoP15I, FokI, SapI, TspRI
Offset cutter
I-CeuI, I-SceI, PI-PspI, PI-SceI, I-PpoI
Homing endonuclease
Nt.BbvCI, Nt.BstNBI, Nt.AlwI
Nicking endonuclease
AgeI, AscI, BamHI, BbvCI, FseI, HindIII, KasI, NcoI, NdeI, NgoMIV, PacI, PmeI (MssI), RsrII, SacI, SalI, SfiI, SgfI, SgrAI, SrfI, SwaI (SmiI), XcmI, XhoI, XmaI, XmnI, ZraI
Restriction endonuclease

A list of endonuclease sites targeted for removal from BioBrick vectors parts. The endonuclease sites were targeted for removal to enable various end-user DNA cloning and manipulation applications.

Shetty et al. Journal of Biological Engineering 2008 2:5   doi:10.1186/1754-1611-2-5