Figure 3.

Representative Slot Blots. Chemiluminescence detection of Fn on control nitrocellulose (Lane A), corresponding image of oxidized Lumigen reaction product (B). Chemiluminescence detection of Fn on electrospun nitrocellulose (Lane C), corresponding image of oxidized Lumigen reaction product (D). Graphical illustration depicting the relative optical density present in slot blots (E). Conventional nitrocellulose blot exhibited modest increase in chemiluminescence signal as a function of increasing Fn concentration (R² = 0.655). Electrospun nitrocellulose exhibited a more pronounced signal at all protein concentrations examined. Signal increased in a nearly linear fashion over a broad range of concentrations (R² = 0.905).